Sensitivity of isolated strains from Far Eastern natural populations rhizobia, to antimicrobials
نویسندگان
چکیده
Relevance . 76 rhizobia strains differing in their physiological and biochemical characteristics from B japonicum S. fredii were isolated the root nodules of Vigna radiate , unguiculata angularis taken soils Far East Russian Federation, left for further research. Methods The virulence new into a pure culture various leguminous crops was determined by growing bacterized seeds test tubes (height — 200 mm, diameter 20 mm) with nutrient medium plants following composition, g/l: K 2 НРО 4 1.0; МgSO CaSO 0.5; FeSO H 3 BO MnSO (NН ) 6 Мо 7 О 24 traces. nodule presence; intensity formation due to strain studied number. Primary assessment inherent resistance antibiotics performed disk diffusion test. Antibiotics used research: nalidixic acid (30 μg), carbenicillin (100 streptomycin (10 erythromycin (15 rifampicin (5 tetracycline μg). After days incubation at temperature +27…28 °C, results registered according growth suppression zone: up 10 mm resistant (R); 15 intermediary (I); 25 sensitive (S); over highly (HS). Results It found that most streptomycin, erythromycin, rifampicin, acid, while highest sensitivity noted carbenicillin. Strains can be attributed Bradyrhizobium elkanii species.
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ژورنال
عنوان ژورنال: Agrarnaâ nauka
سال: 2023
ISSN: ['0869-8155', '2686-701X']
DOI: https://doi.org/10.32634/0869-8155-2023-366-1-70-75